Reformation of the Nigrostriatal Pathway by Fetal Dopaminergic Micrografts into the Substantia Nigra Is Critically Dependent on the Age of the Host
Identifieur interne : 002E18 ( Main/Corpus ); précédent : 002E17; suivant : 002E19Reformation of the Nigrostriatal Pathway by Fetal Dopaminergic Micrografts into the Substantia Nigra Is Critically Dependent on the Age of the Host
Auteurs : Claas Bentlage ; Guido Nikkhah ; Miles G. Cunningham ; Anders BjörklundSource :
- Experimental Neurology [ 0014-4886 ] ; 1999.
English descriptors
- KwdEn :
Abstract
The aim of this study was to determine whether the growth of axons along the nigrostriatal pathway from fetal dopamine cells, transplanted into the substantia nigra of young postnatal 6-OHDA-lesioned rats, is dependent on the age of the host brain. Neonatal rats were lesioned bilaterally by intraventricular injection of 6-OHDA at postnatal day 1 (P1) and received grafts of E14 ventral mesencephalon at day 3 (group P3), day 10 (group P10), or day 20 (group P20) into the right substantia nigra. One lesioned group was left untransplanted. Six months after surgery the animals were subjected to analysis of drug-induced rotation following injection of amphetamine, apomorphine, a D1 agonist (SKF38393), or a D2 agonist (Quinpirole). Animals transplanted intranigrally at day 3 and day 10 showed a strong amphetamine-induced rotational bias toward the side contralateral to the transplant. Animals transplanted into substantia nigra at P20, like the lesioned control animals, showed no rotational bias. Apomorphine and selective D1 and D2 agonists induced ipsilateral turning behavior in the P3 and P10 group, but not in the P20 or the lesion control groups. Immunofluorescence histochemistry in combination with retrograde axonal tracing, using FluoroGold injection into the ipsilateral caudate-putamen showed colocalization of tyrosine hydroxylase and FluoroGold in large numbers of transplanted neurons in the animals transplanted at postnatal day 3 and postnatal day 10, which was not observed in the group P20. The lesion control group showed a 90% complete lesion of the TH-positive cells in the substantia nigra while largely sparing the neurons in the ventral tegmental area. The results indicate that intranigral grafts can be placed accurately and survive well within the substantia nigra region at various time points during postnatal development. Furthermore, embryonic dopamine neurons have the ability to extend axons along the nigrostriatal pathway and reconnect with the dopamine-depleted striatum when transplanted at postnatal day 3 and postnatal day 10, but not at postnatal day 20.
Url:
DOI: 10.1006/exnr.1999.7110
Links to Exploration step
ISTEX:D5D4E3488CB780DC73B4F8F8177E51A248EB6892Le document en format XML
<record><TEI wicri:istexFullTextTei="biblStruct"><teiHeader><fileDesc><titleStmt><title xml:lang="en">Reformation of the Nigrostriatal Pathway by Fetal Dopaminergic Micrografts into the Substantia Nigra Is Critically Dependent on the Age of the Host</title><author><name sortKey="Bentlage, Claas" sort="Bentlage, Claas" uniqKey="Bentlage C" first="Claas" last="Bentlage">Claas Bentlage</name><affiliation><mods:affiliation>Department of Physiology, Wallenberg Neuroscience Center, Sölvegatan 17, S-223 62, Lund, Sweden</mods:affiliation></affiliation></author><author><name sortKey="Nikkhah, Guido" sort="Nikkhah, Guido" uniqKey="Nikkhah G" first="Guido" last="Nikkhah">Guido Nikkhah</name><affiliation><mods:affiliation>Department of Physiology, Wallenberg Neuroscience Center, Sölvegatan 17, S-223 62, Lund, Sweden</mods:affiliation></affiliation></author><author><name sortKey="Cunningham, Miles G" sort="Cunningham, Miles G" uniqKey="Cunningham M" first="Miles G." last="Cunningham">Miles G. Cunningham</name><affiliation><mods:affiliation>Harvard Medical School, 28 Harvard, Charleston, Massachusetts 02129</mods:affiliation></affiliation></author><author><name sortKey="Bjorklund, Anders" sort="Bjorklund, Anders" uniqKey="Bjorklund A" first="Anders" last="Björklund">Anders Björklund</name><affiliation><mods:affiliation>Department of Physiology, Wallenberg Neuroscience Center, Sölvegatan 17, S-223 62, Lund, Sweden</mods:affiliation></affiliation></author></titleStmt><publicationStmt><idno type="wicri:source">ISTEX</idno><idno type="RBID">ISTEX:D5D4E3488CB780DC73B4F8F8177E51A248EB6892</idno><date when="1999" year="1999">1999</date><idno type="doi">10.1006/exnr.1999.7110</idno><idno type="url">https://api.istex.fr/document/D5D4E3488CB780DC73B4F8F8177E51A248EB6892/fulltext/pdf</idno><idno type="wicri:Area/Main/Corpus">002E18</idno></publicationStmt><sourceDesc><biblStruct><analytic><title level="a" type="main" xml:lang="en">Reformation of the Nigrostriatal Pathway by Fetal Dopaminergic Micrografts into the Substantia Nigra Is Critically Dependent on the Age of the Host</title><author><name sortKey="Bentlage, Claas" sort="Bentlage, Claas" uniqKey="Bentlage C" first="Claas" last="Bentlage">Claas Bentlage</name><affiliation><mods:affiliation>Department of Physiology, Wallenberg Neuroscience Center, Sölvegatan 17, S-223 62, Lund, Sweden</mods:affiliation></affiliation></author><author><name sortKey="Nikkhah, Guido" sort="Nikkhah, Guido" uniqKey="Nikkhah G" first="Guido" last="Nikkhah">Guido Nikkhah</name><affiliation><mods:affiliation>Department of Physiology, Wallenberg Neuroscience Center, Sölvegatan 17, S-223 62, Lund, Sweden</mods:affiliation></affiliation></author><author><name sortKey="Cunningham, Miles G" sort="Cunningham, Miles G" uniqKey="Cunningham M" first="Miles G." last="Cunningham">Miles G. Cunningham</name><affiliation><mods:affiliation>Harvard Medical School, 28 Harvard, Charleston, Massachusetts 02129</mods:affiliation></affiliation></author><author><name sortKey="Bjorklund, Anders" sort="Bjorklund, Anders" uniqKey="Bjorklund A" first="Anders" last="Björklund">Anders Björklund</name><affiliation><mods:affiliation>Department of Physiology, Wallenberg Neuroscience Center, Sölvegatan 17, S-223 62, Lund, Sweden</mods:affiliation></affiliation></author></analytic><monogr></monogr><series><title level="j">Experimental Neurology</title><title level="j" type="abbrev">YEXNR</title><idno type="ISSN">0014-4886</idno><imprint><publisher>ELSEVIER</publisher><date type="published" when="1999">1999</date><biblScope unit="volume">159</biblScope><biblScope unit="issue">1</biblScope><biblScope unit="page" from="177">177</biblScope><biblScope unit="page" to="190">190</biblScope></imprint><idno type="ISSN">0014-4886</idno></series><idno type="istex">D5D4E3488CB780DC73B4F8F8177E51A248EB6892</idno><idno type="DOI">10.1006/exnr.1999.7110</idno><idno type="PII">S0014-4886(99)97110-8</idno></biblStruct></sourceDesc><seriesStmt><idno type="ISSN">0014-4886</idno></seriesStmt></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>6-hydroxydopamine</term><term>Neural transplantation</term><term>Parkinson</term><term>dopamine</term><term>fiber outgrowth</term><term>neonatal rats</term><term>striatum.</term><term>substantia nigra</term></keywords></textClass><langUsage><language ident="en">en</language></langUsage></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">The aim of this study was to determine whether the growth of axons along the nigrostriatal pathway from fetal dopamine cells, transplanted into the substantia nigra of young postnatal 6-OHDA-lesioned rats, is dependent on the age of the host brain. Neonatal rats were lesioned bilaterally by intraventricular injection of 6-OHDA at postnatal day 1 (P1) and received grafts of E14 ventral mesencephalon at day 3 (group P3), day 10 (group P10), or day 20 (group P20) into the right substantia nigra. One lesioned group was left untransplanted. Six months after surgery the animals were subjected to analysis of drug-induced rotation following injection of amphetamine, apomorphine, a D1 agonist (SKF38393), or a D2 agonist (Quinpirole). Animals transplanted intranigrally at day 3 and day 10 showed a strong amphetamine-induced rotational bias toward the side contralateral to the transplant. Animals transplanted into substantia nigra at P20, like the lesioned control animals, showed no rotational bias. Apomorphine and selective D1 and D2 agonists induced ipsilateral turning behavior in the P3 and P10 group, but not in the P20 or the lesion control groups. Immunofluorescence histochemistry in combination with retrograde axonal tracing, using FluoroGold injection into the ipsilateral caudate-putamen showed colocalization of tyrosine hydroxylase and FluoroGold in large numbers of transplanted neurons in the animals transplanted at postnatal day 3 and postnatal day 10, which was not observed in the group P20. The lesion control group showed a 90% complete lesion of the TH-positive cells in the substantia nigra while largely sparing the neurons in the ventral tegmental area. The results indicate that intranigral grafts can be placed accurately and survive well within the substantia nigra region at various time points during postnatal development. Furthermore, embryonic dopamine neurons have the ability to extend axons along the nigrostriatal pathway and reconnect with the dopamine-depleted striatum when transplanted at postnatal day 3 and postnatal day 10, but not at postnatal day 20.</div></front></TEI><istex><corpusName>elsevier</corpusName><author><json:item><name>Claas Bentlage</name><affiliations><json:string>Department of Physiology, Wallenberg Neuroscience Center, Sölvegatan 17, S-223 62, Lund, Sweden</json:string></affiliations></json:item><json:item><name>Guido Nikkhah</name><affiliations><json:string>Department of Physiology, Wallenberg Neuroscience Center, Sölvegatan 17, S-223 62, Lund, Sweden</json:string></affiliations></json:item><json:item><name>Miles G. Cunningham</name><affiliations><json:string>Harvard Medical School, 28 Harvard, Charleston, Massachusetts 02129</json:string></affiliations></json:item><json:item><name>Anders Björklund</name><affiliations><json:string>Department of Physiology, Wallenberg Neuroscience Center, Sölvegatan 17, S-223 62, Lund, Sweden</json:string></affiliations></json:item></author><subject><json:item><lang><json:string>eng</json:string></lang><value>Neural transplantation</value></json:item><json:item><lang><json:string>eng</json:string></lang><value>neonatal rats</value></json:item><json:item><lang><json:string>eng</json:string></lang><value>fiber outgrowth</value></json:item><json:item><lang><json:string>eng</json:string></lang><value>dopamine</value></json:item><json:item><lang><json:string>eng</json:string></lang><value>6-hydroxydopamine</value></json:item><json:item><lang><json:string>eng</json:string></lang><value>Parkinson</value></json:item><json:item><lang><json:string>eng</json:string></lang><value>substantia nigra</value></json:item><json:item><lang><json:string>eng</json:string></lang><value>striatum.</value></json:item></subject><language><json:string>eng</json:string></language><abstract>The aim of this study was to determine whether the growth of axons along the nigrostriatal pathway from fetal dopamine cells, transplanted into the substantia nigra of young postnatal 6-OHDA-lesioned rats, is dependent on the age of the host brain. Neonatal rats were lesioned bilaterally by intraventricular injection of 6-OHDA at postnatal day 1 (P1) and received grafts of E14 ventral mesencephalon at day 3 (group P3), day 10 (group P10), or day 20 (group P20) into the right substantia nigra. One lesioned group was left untransplanted. Six months after surgery the animals were subjected to analysis of drug-induced rotation following injection of amphetamine, apomorphine, a D1 agonist (SKF38393), or a D2 agonist (Quinpirole). Animals transplanted intranigrally at day 3 and day 10 showed a strong amphetamine-induced rotational bias toward the side contralateral to the transplant. Animals transplanted into substantia nigra at P20, like the lesioned control animals, showed no rotational bias. Apomorphine and selective D1 and D2 agonists induced ipsilateral turning behavior in the P3 and P10 group, but not in the P20 or the lesion control groups. Immunofluorescence histochemistry in combination with retrograde axonal tracing, using FluoroGold injection into the ipsilateral caudate-putamen showed colocalization of tyrosine hydroxylase and FluoroGold in large numbers of transplanted neurons in the animals transplanted at postnatal day 3 and postnatal day 10, which was not observed in the group P20. The lesion control group showed a 90% complete lesion of the TH-positive cells in the substantia nigra while largely sparing the neurons in the ventral tegmental area. The results indicate that intranigral grafts can be placed accurately and survive well within the substantia nigra region at various time points during postnatal development. Furthermore, embryonic dopamine neurons have the ability to extend axons along the nigrostriatal pathway and reconnect with the dopamine-depleted striatum when transplanted at postnatal day 3 and postnatal day 10, but not at postnatal day 20.</abstract><qualityIndicators><score>8</score><pdfVersion>1.2</pdfVersion><pdfPageSize>554 x 734 pts</pdfPageSize><refBibsNative>true</refBibsNative><keywordCount>8</keywordCount><abstractCharCount>2102</abstractCharCount><pdfWordCount>6321</pdfWordCount><pdfCharCount>39550</pdfCharCount><pdfPageCount>14</pdfPageCount><abstractWordCount>315</abstractWordCount></qualityIndicators><title>Reformation of the Nigrostriatal Pathway by Fetal Dopaminergic Micrografts into the Substantia Nigra Is Critically Dependent on the Age of the Host</title><pii><json:string>S0014-4886(99)97110-8</json:string></pii><genre><json:string>research-article</json:string></genre><host><volume>159</volume><pii><json:string>S0014-4886(00)X0028-3</json:string></pii><pages><last>190</last><first>177</first></pages><issn><json:string>0014-4886</json:string></issn><issue>1</issue><genre><json:string>Journal</json:string></genre><language><json:string>unknown</json:string></language><title>Experimental Neurology</title><publicationDate>1999</publicationDate></host><categories><wos><json:string>NEUROSCIENCES</json:string></wos></categories><publicationDate>1999</publicationDate><copyrightDate>1999</copyrightDate><doi><json:string>10.1006/exnr.1999.7110</json:string></doi><id>D5D4E3488CB780DC73B4F8F8177E51A248EB6892</id><fulltext><json:item><original>true</original><mimetype>application/pdf</mimetype><extension>pdf</extension><uri>https://api.istex.fr/document/D5D4E3488CB780DC73B4F8F8177E51A248EB6892/fulltext/pdf</uri></json:item><json:item><original>false</original><mimetype>application/zip</mimetype><extension>zip</extension><uri>https://api.istex.fr/document/D5D4E3488CB780DC73B4F8F8177E51A248EB6892/fulltext/zip</uri></json:item><istex:fulltextTEI uri="https://api.istex.fr/document/D5D4E3488CB780DC73B4F8F8177E51A248EB6892/fulltext/tei"><teiHeader><fileDesc><titleStmt><title level="a" type="main" xml:lang="en">Reformation of the Nigrostriatal Pathway by Fetal Dopaminergic Micrografts into the Substantia Nigra Is Critically Dependent on the Age of the Host</title></titleStmt><publicationStmt><authority>ISTEX</authority><publisher>ELSEVIER</publisher><availability><p>ELSEVIER</p></availability><date>1999</date></publicationStmt><notesStmt><note type="content">Section title: Regular Article</note></notesStmt><sourceDesc><biblStruct type="inbook"><analytic><title level="a" type="main" xml:lang="en">Reformation of the Nigrostriatal Pathway by Fetal Dopaminergic Micrografts into the Substantia Nigra Is Critically Dependent on the Age of the Host</title><author><persName><forename type="first">Claas</forename><surname>Bentlage</surname></persName><affiliation>Department of Physiology, Wallenberg Neuroscience Center, Sölvegatan 17, S-223 62, Lund, Sweden</affiliation></author><author><persName><forename type="first">Guido</forename><surname>Nikkhah</surname></persName><affiliation>Department of Physiology, Wallenberg Neuroscience Center, Sölvegatan 17, S-223 62, Lund, Sweden</affiliation></author><author><persName><forename type="first">Miles G.</forename><surname>Cunningham</surname></persName><affiliation>Harvard Medical School, 28 Harvard, Charleston, Massachusetts 02129</affiliation></author><author><persName><forename type="first">Anders</forename><surname>Björklund</surname></persName><affiliation>Department of Physiology, Wallenberg Neuroscience Center, Sölvegatan 17, S-223 62, Lund, Sweden</affiliation></author></analytic><monogr><title level="j">Experimental Neurology</title><title level="j" type="abbrev">YEXNR</title><idno type="pISSN">0014-4886</idno><idno type="PII">S0014-4886(00)X0028-3</idno><imprint><publisher>ELSEVIER</publisher><date type="published" when="1999"></date><biblScope unit="volume">159</biblScope><biblScope unit="issue">1</biblScope><biblScope unit="page" from="177">177</biblScope><biblScope unit="page" to="190">190</biblScope></imprint></monogr><idno type="istex">D5D4E3488CB780DC73B4F8F8177E51A248EB6892</idno><idno type="DOI">10.1006/exnr.1999.7110</idno><idno type="PII">S0014-4886(99)97110-8</idno></biblStruct></sourceDesc></fileDesc><profileDesc><creation><date>1999</date></creation><langUsage><language ident="en">en</language></langUsage><abstract xml:lang="en"><p>The aim of this study was to determine whether the growth of axons along the nigrostriatal pathway from fetal dopamine cells, transplanted into the substantia nigra of young postnatal 6-OHDA-lesioned rats, is dependent on the age of the host brain. Neonatal rats were lesioned bilaterally by intraventricular injection of 6-OHDA at postnatal day 1 (P1) and received grafts of E14 ventral mesencephalon at day 3 (group P3), day 10 (group P10), or day 20 (group P20) into the right substantia nigra. One lesioned group was left untransplanted. Six months after surgery the animals were subjected to analysis of drug-induced rotation following injection of amphetamine, apomorphine, a D1 agonist (SKF38393), or a D2 agonist (Quinpirole). Animals transplanted intranigrally at day 3 and day 10 showed a strong amphetamine-induced rotational bias toward the side contralateral to the transplant. Animals transplanted into substantia nigra at P20, like the lesioned control animals, showed no rotational bias. Apomorphine and selective D1 and D2 agonists induced ipsilateral turning behavior in the P3 and P10 group, but not in the P20 or the lesion control groups. Immunofluorescence histochemistry in combination with retrograde axonal tracing, using FluoroGold injection into the ipsilateral caudate-putamen showed colocalization of tyrosine hydroxylase and FluoroGold in large numbers of transplanted neurons in the animals transplanted at postnatal day 3 and postnatal day 10, which was not observed in the group P20. The lesion control group showed a 90% complete lesion of the TH-positive cells in the substantia nigra while largely sparing the neurons in the ventral tegmental area. The results indicate that intranigral grafts can be placed accurately and survive well within the substantia nigra region at various time points during postnatal development. Furthermore, embryonic dopamine neurons have the ability to extend axons along the nigrostriatal pathway and reconnect with the dopamine-depleted striatum when transplanted at postnatal day 3 and postnatal day 10, but not at postnatal day 20.</p></abstract><textClass xml:lang="en"><keywords scheme="keyword"><list><head>Keywords</head><item><term>Neural transplantation</term></item><item><term>neonatal rats</term></item><item><term>fiber outgrowth</term></item><item><term>dopamine</term></item><item><term>6-hydroxydopamine</term></item><item><term>Parkinson</term></item><item><term>substantia nigra</term></item><item><term>striatum.</term></item></list></keywords></textClass></profileDesc><revisionDesc><change when="1999-04-09">Registration</change><change when="1999">Published</change></revisionDesc></teiHeader></istex:fulltextTEI><json:item><original>false</original><mimetype>text/plain</mimetype><extension>txt</extension><uri>https://api.istex.fr/document/D5D4E3488CB780DC73B4F8F8177E51A248EB6892/fulltext/txt</uri></json:item></fulltext><metadata><istex:metadataXml wicri:clean="Elsevier, elements deleted: tail"><istex:xmlDeclaration>version="1.0" encoding="utf-8"</istex:xmlDeclaration><istex:docType PUBLIC="-//ES//DTD journal article DTD version 4.5.2//EN//XML" URI="art452.dtd" name="istex:docType"></istex:docType><istex:document><converted-article version="4.5.2" docsubtype="fla" xml:lang="en"><item-info><jid>YEXNR</jid><aid>97110</aid><ce:pii>S0014-4886(99)97110-8</ce:pii><ce:doi>10.1006/exnr.1999.7110</ce:doi><ce:copyright type="full-transfer" year="1999">Academic Press</ce:copyright></item-info><head><ce:dochead><ce:textfn>Regular Article</ce:textfn></ce:dochead><ce:title>Reformation of the Nigrostriatal Pathway by Fetal Dopaminergic Micrografts into the Substantia Nigra Is Critically Dependent on the Age of the Host</ce:title><ce:author-group><ce:author><ce:given-name>Claas</ce:given-name><ce:surname>Bentlage</ce:surname><ce:cross-ref refid="A1"><ce:sup>a</ce:sup></ce:cross-ref><ce:cross-ref refid="A2"><ce:sup>b</ce:sup></ce:cross-ref></ce:author><ce:author><ce:given-name>Guido</ce:given-name><ce:surname>Nikkhah</ce:surname><ce:cross-ref refid="A1"><ce:sup>a</ce:sup></ce:cross-ref><ce:cross-ref refid="A2"><ce:sup>b</ce:sup></ce:cross-ref></ce:author><ce:author><ce:given-name>Miles G.</ce:given-name><ce:surname>Cunningham</ce:surname><ce:cross-ref refid="A3"><ce:sup>c</ce:sup></ce:cross-ref></ce:author><ce:author><ce:given-name>Anders</ce:given-name><ce:surname>Björklund</ce:surname><ce:cross-ref refid="A1"><ce:sup>a</ce:sup></ce:cross-ref></ce:author><ce:affiliation id="A1"><ce:label>a</ce:label><ce:textfn>Department of Physiology, Wallenberg Neuroscience Center, Sölvegatan 17, S-223 62, Lund, Sweden</ce:textfn></ce:affiliation><ce:affiliation id="A2"><ce:label>b</ce:label><ce:textfn>Neurosurgical Clinic, Nordstadt Hospital, Haltenhofstrasse, 41, D-30167, Hannover, Germany</ce:textfn></ce:affiliation><ce:affiliation id="A3"><ce:label>c</ce:label><ce:textfn>Harvard Medical School, 28 Harvard, Charleston, Massachusetts 02129</ce:textfn></ce:affiliation></ce:author-group><ce:date-received day="21" month="9" year="1998"></ce:date-received><ce:date-accepted day="9" month="4" year="1999"></ce:date-accepted><ce:abstract><ce:section-title>Abstract</ce:section-title><ce:abstract-sec><ce:simple-para>The aim of this study was to determine whether the growth of axons along the nigrostriatal pathway from fetal dopamine cells, transplanted into the substantia nigra of young postnatal 6-OHDA-lesioned rats, is dependent on the age of the host brain. Neonatal rats were lesioned bilaterally by intraventricular injection of 6-OHDA at postnatal day 1 (P1) and received grafts of E14 ventral mesencephalon at day 3 (group P3), day 10 (group P10), or day 20 (group P20) into the right substantia nigra. One lesioned group was left untransplanted. Six months after surgery the animals were subjected to analysis of drug-induced rotation following injection of amphetamine, apomorphine, a D1 agonist (SKF38393), or a D2 agonist (Quinpirole). Animals transplanted intranigrally at day 3 and day 10 showed a strong amphetamine-induced rotational bias toward the side contralateral to the transplant. Animals transplanted into substantia nigra at P20, like the lesioned control animals, showed no rotational bias. Apomorphine and selective D1 and D2 agonists induced ipsilateral turning behavior in the P3 and P10 group, but not in the P20 or the lesion control groups. Immunofluorescence histochemistry in combination with retrograde axonal tracing, using FluoroGold injection into the ipsilateral caudate-putamen showed colocalization of tyrosine hydroxylase and FluoroGold in large numbers of transplanted neurons in the animals transplanted at postnatal day 3 and postnatal day 10, which was not observed in the group P20. The lesion control group showed a 90% complete lesion of the TH-positive cells in the substantia nigra while largely sparing the neurons in the ventral tegmental area. The results indicate that intranigral grafts can be placed accurately and survive well within the substantia nigra region at various time points during postnatal development. Furthermore, embryonic dopamine neurons have the ability to extend axons along the nigrostriatal pathway and reconnect with the dopamine-depleted striatum when transplanted at postnatal day 3 and postnatal day 10, but not at postnatal day 20.</ce:simple-para></ce:abstract-sec></ce:abstract><ce:keywords><ce:section-title>Keywords</ce:section-title><ce:keyword><ce:text>Neural transplantation</ce:text></ce:keyword><ce:keyword><ce:text>neonatal rats</ce:text></ce:keyword><ce:keyword><ce:text>fiber outgrowth</ce:text></ce:keyword><ce:keyword><ce:text>dopamine</ce:text></ce:keyword><ce:keyword><ce:text>6-hydroxydopamine</ce:text></ce:keyword><ce:keyword><ce:text>Parkinson</ce:text></ce:keyword><ce:keyword><ce:text>substantia nigra</ce:text></ce:keyword><ce:keyword><ce:text>striatum.</ce:text></ce:keyword></ce:keywords></head></converted-article></istex:document></istex:metadataXml><mods version="3.6"><titleInfo lang="en"><title>Reformation of the Nigrostriatal Pathway by Fetal Dopaminergic Micrografts into the Substantia Nigra Is Critically Dependent on the Age of the Host</title></titleInfo><titleInfo type="alternative" lang="en" contentType="CDATA"><title>Reformation of the Nigrostriatal Pathway by Fetal Dopaminergic Micrografts into the Substantia Nigra Is Critically Dependent on the Age of the Host</title></titleInfo><name type="personal"><namePart type="given">Claas</namePart><namePart type="family">Bentlage</namePart><affiliation>Department of Physiology, Wallenberg Neuroscience Center, Sölvegatan 17, S-223 62, Lund, Sweden</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">Guido</namePart><namePart type="family">Nikkhah</namePart><affiliation>Department of Physiology, Wallenberg Neuroscience Center, Sölvegatan 17, S-223 62, Lund, Sweden</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">Miles G.</namePart><namePart type="family">Cunningham</namePart><affiliation>Harvard Medical School, 28 Harvard, Charleston, Massachusetts 02129</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">Anders</namePart><namePart type="family">Björklund</namePart><affiliation>Department of Physiology, Wallenberg Neuroscience Center, Sölvegatan 17, S-223 62, Lund, Sweden</affiliation><role><roleTerm type="text">author</roleTerm></role></name><typeOfResource>text</typeOfResource><genre type="research-article" displayLabel="Full-length article"></genre><originInfo><publisher>ELSEVIER</publisher><dateIssued encoding="w3cdtf">1999</dateIssued><dateValid encoding="w3cdtf">1999-04-09</dateValid><copyrightDate encoding="w3cdtf">1999</copyrightDate></originInfo><language><languageTerm type="code" authority="iso639-2b">eng</languageTerm><languageTerm type="code" authority="rfc3066">en</languageTerm></language><physicalDescription><internetMediaType>text/html</internetMediaType></physicalDescription><abstract lang="en">The aim of this study was to determine whether the growth of axons along the nigrostriatal pathway from fetal dopamine cells, transplanted into the substantia nigra of young postnatal 6-OHDA-lesioned rats, is dependent on the age of the host brain. Neonatal rats were lesioned bilaterally by intraventricular injection of 6-OHDA at postnatal day 1 (P1) and received grafts of E14 ventral mesencephalon at day 3 (group P3), day 10 (group P10), or day 20 (group P20) into the right substantia nigra. One lesioned group was left untransplanted. Six months after surgery the animals were subjected to analysis of drug-induced rotation following injection of amphetamine, apomorphine, a D1 agonist (SKF38393), or a D2 agonist (Quinpirole). Animals transplanted intranigrally at day 3 and day 10 showed a strong amphetamine-induced rotational bias toward the side contralateral to the transplant. Animals transplanted into substantia nigra at P20, like the lesioned control animals, showed no rotational bias. Apomorphine and selective D1 and D2 agonists induced ipsilateral turning behavior in the P3 and P10 group, but not in the P20 or the lesion control groups. Immunofluorescence histochemistry in combination with retrograde axonal tracing, using FluoroGold injection into the ipsilateral caudate-putamen showed colocalization of tyrosine hydroxylase and FluoroGold in large numbers of transplanted neurons in the animals transplanted at postnatal day 3 and postnatal day 10, which was not observed in the group P20. The lesion control group showed a 90% complete lesion of the TH-positive cells in the substantia nigra while largely sparing the neurons in the ventral tegmental area. The results indicate that intranigral grafts can be placed accurately and survive well within the substantia nigra region at various time points during postnatal development. Furthermore, embryonic dopamine neurons have the ability to extend axons along the nigrostriatal pathway and reconnect with the dopamine-depleted striatum when transplanted at postnatal day 3 and postnatal day 10, but not at postnatal day 20.</abstract><note type="content">Section title: Regular Article</note><subject lang="en"><genre>Keywords</genre><topic>Neural transplantation</topic><topic>neonatal rats</topic><topic>fiber outgrowth</topic><topic>dopamine</topic><topic>6-hydroxydopamine</topic><topic>Parkinson</topic><topic>substantia nigra</topic><topic>striatum.</topic></subject><relatedItem type="host"><titleInfo><title>Experimental Neurology</title></titleInfo><titleInfo type="abbreviated"><title>YEXNR</title></titleInfo><genre type="Journal">journal</genre><originInfo><dateIssued encoding="w3cdtf">199909</dateIssued></originInfo><identifier type="ISSN">0014-4886</identifier><identifier type="PII">S0014-4886(00)X0028-3</identifier><part><date>199909</date><detail type="volume"><number>159</number><caption>vol.</caption></detail><detail type="issue"><number>1</number><caption>no.</caption></detail><extent unit="issue pages"><start>1</start><end>331</end></extent><extent unit="pages"><start>177</start><end>190</end></extent></part></relatedItem><identifier type="istex">D5D4E3488CB780DC73B4F8F8177E51A248EB6892</identifier><identifier type="DOI">10.1006/exnr.1999.7110</identifier><identifier type="PII">S0014-4886(99)97110-8</identifier><accessCondition type="use and reproduction" contentType="">© 1999Academic Press</accessCondition><recordInfo><recordContentSource>ELSEVIER</recordContentSource><recordOrigin>Academic Press, ©1999</recordOrigin></recordInfo></mods></metadata><enrichments><istex:catWosTEI uri="https://api.istex.fr/document/D5D4E3488CB780DC73B4F8F8177E51A248EB6892/enrichments/catWos"><teiHeader><profileDesc><textClass><classCode scheme="WOS">NEUROSCIENCES</classCode></textClass></profileDesc></teiHeader></istex:catWosTEI></enrichments><serie></serie></istex></record>Pour manipuler ce document sous Unix (Dilib)
EXPLOR_STEP=$WICRI_ROOT/Wicri/Sante/explor/ParkinsonV1/Data/Main/Corpus
HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 002E18 | SxmlIndent | more
Ou
HfdSelect -h $EXPLOR_AREA/Data/Main/Corpus/biblio.hfd -nk 002E18 | SxmlIndent | more
Pour mettre un lien sur cette page dans le réseau Wicri
{{Explor lien
|wiki= Wicri/Sante
|area= ParkinsonV1
|flux= Main
|étape= Corpus
|type= RBID
|clé= ISTEX:D5D4E3488CB780DC73B4F8F8177E51A248EB6892
|texte= Reformation of the Nigrostriatal Pathway by Fetal Dopaminergic Micrografts into the Substantia Nigra Is Critically Dependent on the Age of the Host
}}
| This area was generated with Dilib version V0.6.23. |  |